A GENETIC TOOLBOX FOR THE IDENTIFICATION AND INTERROGATION OF TUMOR CELLS OF ORIGIN, PROPAGATION, AND RECURRENCE FROM PATIENT-DERIVED ONCOGENIC DRIVERS


Year of Award:
2016
Award Type:
R33
Project Number:
1R33CA202900-01
RFA Number:
RFA-CA-15-003
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
BREUNIG, JOSHUA JOHN
Other PI or Project Leader:
Not Applicable
Institution:
CEDARS-SINAI MEDICAL CENTER
To further develop and validate a technique for generating genetically engineered mouse models which has previously been demonstrated for neurology and that allows for multiple driver mutations to arise and be studied in combination. This modular transposon-based system involves delivering a single transposable vector by perinatal electroporation to rapidly develop various combinatorial models and they will demonstrate a capability for quickly obtaining mice that have combinations of known mutations associated with glioblastoma.