ISOLATION AND IN VITRO MAINTENANCE OF INTACT COLONIC CRYPTS FROM NORMAL HUMAN COL


Year of Award:
2014
Award Type:
R21
Project Number:
CA181855
RFA Number:
RFA-CA-13-003
Technology Track:
Biospecimen Science Technologies
PI/Project Leader:
VARANI, JAMES
Other PI or Project Leader:
N/A
Institution:
UNIVERSITY OF MICHIGAN
Abstract the long-term goal of the research proposed here is to develop methods for the isolation and in vitro maintenance of intact colonic crypts from histologically normal human colon tissue and from adenomatous polyps / malignant tumors. The isolated