LABEL FREE IMAGING OF BLOOD SMEARS AND TISSUE BIOPSIES


Year of Award:
2010
Award Type:
R21
Project Number:
CA147967
RFA Number:
RFA-CA-09-004
Technology Track:
Biospecimen Science Technologies
PI/Project Leader:
POPESCU, GABRIEL
Other PI or Project Leader:
N/A
Institution:
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
This exploratory project is aimed at investigating the potential of newly developed optical interferometric technique that we developed- Spatial Light Interference Microscopy (SLIM)-- for characterizing blood smears and tissue biopsies. SLIM is label-free and uniquely sensitive to refractive index modifications brought to the biospecimen by disease development . If successful, in the long-run, i.e. 5-10 years period, we anticipate that this label-free approach to imaging biospecimens will provide faster access to clinically-relevant data, as it bypasses most of the histology preparation process, e.g. staining. Further, due to its exquisite sensitivity to structural changes in the tissue, SLIM will provide a greater wealth of information than common histology, which should aid the pathologist in reaching a faster, more accurate diagnosis. The exploratory stage project will have the following specific aims: develop advanced, high-throughput instrumentation, perform proof-of principle studies on blood smears with focus on several specific diseases, and image biopsies of normal and three types of cancers (breast, prostate, and lung).