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A MOLECULAR METHOD TO DETERMINE ISOFORM FREQUENCIES IN RNA-SEQ


Year of Award:
2015
Status:
Complete
Award Type:
R21
Project Number:
CA196438
RFA Number:
RFA-CA-14-003
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
WELSH, JOHN T
Other PI or Project Leader:
N/A
Institution:
VACCINE RESEARCH INSTITUTE OF SAN DIEGO
Current methods for RNA-seq library preparation attempt to uniformly sample all sequences across every mRNA molecule, optimally with sufficient overlap to allow de novo reassembly of the mRNA sequences from which they derive, or alternatively, to allow in