MOLECULAR DETECTION OF DNA HYDROXYMETHYLATION FOR CANCER SCREENING


Year of Award:
2015
Award Type:
R21
Project Number:
CA193067
RFA Number:
RFA-CA-14-003
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
HALL, ADAM ROGER
Other PI or Project Leader:
N/A
Institution:
WAKE FOREST UNIVERSITY HEALTH SCIENCES
This project will develop a strategy to detect levels of hydroxymethylation (hmC) in genomic DNA. We will first combine a method to selectively attach a biotin to hmC with our demonstrated technique of detecting single biotin modifications in DNA to demonstrate the efficacy of our approach and optimize experimental conditions. We will then apply the technique to clinical tissue samples. First, we will measure the global hmC content of normal breast tissue and of breast carcinoma and compare our findings with results from conventional approaches. Finally, we will utilize the capability of our approach to isolate modified DNA selectively and apply the system to identifying alterations in the hmC content of a fragment of the LZTS1 gene, an important tumor suppressor.