NANOTRAP TECHNOLOGY FOR ONE STEP PRESERVATION AND AMPLIFICATION OF CANCER BIOMARK


Year of Award:
2012
Award Type:
R33
Project Number:
CA173359
RFA Number:
RFA-CA-12-005
Technology Track:
Biospecimen Science Technologies
PI/Project Leader:
LIOTTA, LANCE ALLEN
Other PI or Project Leader:
N/A
Institution:
GEORGE MASON UNIVERSITY
We propose advanced development and validation of novel technology that will maximize the quality and utility of biologic fluid specimens, and permitthe measurement of previously invisible low abundance biomarkers. The technology is transformative and paradigm-shifting because it immediately and economically solves fundamental roadblocks paralyzing the body fluid cancer biomarker field. In a single step, in minutes, our technology overcomes the severe problems of biomarker preservation, low abundance, and masking by unwanted proteins. The technology is novel porous, buoyant, core-shell hydrogel nanoparticles containing high affinity reactive chemical baits that harvests biomarkers in body fluids. Our nanoparticles can be pre loaded into Vacutainer(R) blood collection tubes, or other body fluid collection vessels. Upon contact with the sample, the suspended nanoparticles immediately affinity-sequester target biomarkers inside the particles, exclude albumin, fully protect the biomarkers from degradation (even at elevated temperatures), and massively concentrate the sequestered biomarkers into a small volume. The technology can dramatically (demonstrated up to 10,000 fold) improve the lower limits of detection and the precision of: a) mass spectrometry (MS) biomarker discovery, b) quantitation by multiple reaction monitoring (MRM), or c) quantification by any clinical grade immunoassay. All of the Aims and Milestones of the predicate IMAT NCI R21 CA137706 grant have been exceeded. We have discovered more than a dozen novel chemical baits with preferential high affinities (KD