Year of Award:
Molecular & Cellular Analysis Technologies
Other PI or Project Leader:
PALO ALTO RESEARCH CENTER
The goal of this project is to develop a new calorimetry-based high-throughput drug screening platform. The novelty here lies in the use of thermochromic materials in a microfluidic platform to allow for extremely sensitive, label-free probing for very small enthalpy changes arising from binding or enzymatic reactions. The high sensitivity will extend high-throughput screening to non-enzymatic reactions and possibly allow for probing of novel mechanisms of inhibition and should reduce the volume of reagents needed. The proof of concept will involve demonstrating this platform with well-characterized histone deacetylase inhibitors.