HIGH-THROUGHPUT SCREENING PLATFORM FOR CANCER DRUG DISCOVERY


Year of Award:
2016
Award Type:
R21
Project Number:
1R21CA193074-01A1
RFA Number:
RFA-CA-15-002
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
RECHT, MICHAEL
Other PI or Project Leader:
Not Applicable
Institution:
PALO ALTO RESEARCH CENTER
The goal of this project is to develop a new calorimetry-based high-throughput drug screening platform. The novelty here lies in the use of thermochromic materials in a microfluidic platform to allow for extremely sensitive, label-free probing for very small enthalpy changes arising from binding or enzymatic reactions. The high sensitivity will extend high-throughput screening to non-enzymatic reactions and possibly allow for probing of novel mechanisms of inhibition and should reduce the volume of reagents needed. The proof of concept will involve demonstrating this platform with well-characterized histone deacetylase inhibitors.