VALIDATION OF A ROOM-TEMPERATURE STORAGE TECHNIQUE FOR PLASMA/SERUM BIOSPECIMENS


Year of Award:
2016
Award Type:
R33
Project Number:
1R33CA204510-01
RFA Number:
RFA-CA-15-005
Technology Track:
Biospecimen Science Technologies
PI/Project Leader:
AKSAN, ALPTEKIN
Other PI or Project Leader:
Not Applicable
Institution:
UNIVERSITY OF MINNESOTA
To build upon a successful IMAT R21-supported project to develop a capability for storing liquid biospecimens (mainly, human serum) at room temperature for extensive periods using a vitrification approach with a focus on maintaining the stability of the proteinaceous biomarkers in storage. The approach involves the use of an electrospun lyophilizing sponge that dessicates samples into viscous glassy state. Investigators will further develop the technique to stably preserve a larger array of labile and stable proteins with a substantial validation study showing long term preservation stability of 300+ biomarkers compared across fresh, frozen and lyophilized serum over a 36 month period with two large biorepositories.