ULTRA-BRIGHT PROBES WITH ULTRA-NARROW EMISSION FOR MOLECULAR AND CELLULAR ANALYSI


Year of Award:
2014
Award Type:
R21
Project Number:
CA186798
RFA Number:
RFA-CA-13-001
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
CHIU, DANIEL T
Other PI or Project Leader:
N/A
Institution:
UNIVERSITY OF WASHINGTON
Fluorescence based techniques, from cellular imaging to bioassays, have become an indispensable tool kit in both basic cellular studies of cancer and in clinical applications and in vitro diagnostics. Organic dyes appear to be the most versatile fluorophores used so far in these applications. However, the intrinsic limitations of conventional dyes, such as low absorptivity and poor photostability, have posed difficulties for the further development of high-sensitivity imaging techniques and high-throughput assays. As a result, there has been considerable interest in brighter and more stable fluorescent probes. For example, phycobiliproteins exhibit higher fluorescence brightness than small organic fluorophores, and there is also a great deal of interest in the development of brightly fluorescentnanoparticles, such as semiconductor quantum dots. This project aims to develop a new class of fluorescent probes, called Pdots, for use in the molecular analysis and study of cancer cells. To achieve this goal, we propose the following three aims: Aim 1: Develop multicolor ultra-bright Pdots with ultra-narrow emission bandwidth (Full Width at Half Maximum (FWHM)