A MICRO HALL CHIP FOR CIRCULATING MICROVESICLE BASED CANCER MONITORING


Year of Award:
2014
Award Type:
R21
Project Number:
CA182336
RFA Number:
RFA-CA-13-001
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
ISSADORE, DAVID AARON
Other PI or Project Leader:
N/A
Institution:
UNIVERSITY OF PENNSYLVANIA
Tumor cells are known to shed nano-scale objects called circulating microvesicles (C¨Vs) into patients' blood. These C¨Vs have been shown to carry molecular information from the tumor, which can potentially be used to diagnose and monitor cancer using only a blood test. However, due to their extremely small size (d ~ 50 nm), there has not been a clinically viable method to detect and profile these C¨Vs. To address these challenges, we propose a microchip-based platform that can quantitatively profile C¨Vs directly in unprocessed whole blood. On this chip, we harness the small feature size of microelectronics and combine it with the biocompatibility of microfluidics and magnetic nanoparticles (MNPs) to measure these nano-scale objects. Our proposed device is handheld and aims to reduce measurement times from several hours using conventional equipment to less than thirty minutes. Moreover, due to the high sensitivity of our micro-magnetic sensing method, the biomarker specific limit of detection (100 C¨V/mL) will exceed that of conventional techniques. This innovative, clinically practical approach to C¨V detection has great potential for non-invasive, routine monitoring of disease progression, drug efficacy, and metastasis, offering tremendous benefits for patients.