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FLUORESCENCE IN SITU DETECTION OF SHORT DNA SEQUENCES


Year of Award:
2005
Status:
Complete
Award Type:
R21
Project Number:
CA112418
RFA Number:
RFA-CA-05-002
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
FRANK-KAMENETSKII, MAXIM D
Other PI or Project Leader:
N/A
Institution:
BOSTON UNIVERSITY
A radically new approach for the fluorescence in situ detection of DNA is proposed, which makes it possible to detect short (about 20-bp-long) single-copy DNA sequences in metaphase chromosome spreads and in interphase nuclei under non-denaturing conditio