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DISPLAY METHODS FOR SURVEYING MUTATIONS


Year of Award:
2000
Status:
Complete
Award Type:
R21
Project Number:
CA088326
RFA Number:
PAR-99-100
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
WEISSMAN, SHERMAN MORTON
Other PI or Project Leader:
N/A
Institution:
YALE UNIVERSITY
We propose to develop a gel based approach that would display most of the single base substitution mutations present in any mRNA expressed in a cell type. The method would permit recovery of bands for sequencing and identification of the sites of mutation