GENETIC SELECTION FOR PROTEASES AND THEIR SUBSTRATES


Year of Award:
1999
Award Type:
R43
Project Number:
CA081860
RFA Number:
PAR-98-066
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
CHANG, HWAI W
Other PI or Project Leader:
N/A
Institution:
STRATAGENE
The goal of the proposed research is to develop a genetic method, using the eukaryotic host Saccharomyces cerevisiae, to select for protease genes, identify their target recognition sequences, and select for protease inhibitors. This represents a novel approach in the functional cloning of these important cellular regulators and will enable rapid isolation of their genes, their targets, and their inhibitors. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE