FISH AND CHIPS--SINGLE CELL EXPRESSION OF CANCER GENES


Year of Award:
1999
Award Type:
R21
Project Number:
CA083208
RFA Number:
PAR-98-067
Technology Track:
Molecular & Cellular Analysis Technologies
PI/Project Leader:
SINGER, ROBERT H
Other PI or Project Leader:
N/A
Institution:
YESHIVA UNIVERSITY
The purpose of this proposal is to develop innovative strategies for the detection of multiple gene targets in single tumorous cells. The strategy is to use multicolored detection derived from oligonucleotide probes and combined with deconvolution imaging microscopy to use the entire nucleus of the cell as a chip to display hundreds of gene expression targets. The procedure will then be applied to a colorectal cancer cell model first using human cell lines and proceeding to mouse and eventually human tissue. The benefits of this approach are that many genes can be interrogated for their level of expression in every cell within a tissue, thereby deriving important prognostic and diagnostic information from a single probe cocktail.